Nikon Spinning Disk 2 + Epi

 

The Nikon Spinning Disk 2 + Epifluorescence microscope is an inverted Nikon Ti microscope set up for both spinning disk imaging with a CSU0X1 and EMCCD camera and 4-color fluorescence imaging with and Andor CMOS camera. With a stage top incubator can C02 mixer it can be used for both fixed and live cells. 

 

Links

ILAB CALENDAR

 

 


Capabilities

  • Bright field and DIC imaging
  • 4 color confocal imaging
  • 4-color Epifluorescence imaging
  • Live cell imaging
  • Automated imaging

 


Objectives:

  • Plan Fluor 10x/0.3 NA
  • Plan Apo 20X VC 0.75 NA 
  • Apo 40x 0.95 NA
  • 60x NA 1.40 Oil
  • Plan Apo VC 100x NA 1.40 Oil

Equipment Details:

  • Nikon Ti  Inverted Microscope
  • Hammatsu EMCCD (confocal)
  • Andor CMOS (Widfield)
  • Tokai hit stage top incupator
  • Oko CO2 Mixer
  • Vortan 4 line laser launch
  • Lumencore spectra (LED)

Light Source:

  • Lumencore Spectra (LEDs) EPI
  • Vortan lasers (confocal)
    • 405
    • 488
    • 561
    • 647
 

Emission Filters:

  • multipass zet405-488-561-640m
  • 450-50m
  • 525-50m
  • 610-75m
  • 655lp