Nico Stuurman, PhD

Director of CALM, Associate Adjunct Professor
Biochemistry and Biophysics
Publications: 

Nucleolar dynamics are determined by the ordered assembly of the ribosome.

bioRxiv : the preprint server for biology

Sheu-Gruttadauria J, Yan X, Stuurman N, Floor SN, Vale RD

Three-color single-molecule imaging reveals conformational dynamics of dynein undergoing motility.

Proceedings of the National Academy of Sciences of the United States of America

Niekamp S, Stuurman N, Zhang N, Vale RD

Pycro-Manager: open-source software for customized and reproducible microscope control.

Nature methods

Pinkard H, Stuurman N, Ivanov IE, Anthony NM, Ouyang W, Li B, Yang B, Tsuchida MA, Chhun B, Zhang G, Mei R, Anderson M, Shepherd DP, Hunt-Isaak I, Dunn RL, Jahr W, Kato S, Royer LA, Thiagarajah JR, Eliceiri KW, Lundberg E, Mehta SB, Waller L

High-content imaging-based pooled CRISPR screens in mammalian cells.

The Journal of cell biology

Yan X, Stuurman N, Ribeiro SA, Tanenbaum ME, Horlbeck MA, Liem CR, Jost M, Weissman JS, Vale RD

A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging.

Nature methods

Niekamp S, Stuurman N, Vale RD

Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution.

Nature methods

Yang B, Chen X, Wang Y, Feng S, Pessino V, Stuurman N, Cho NH, Cheng KW, Lord SJ, Xu L, Xie D, Mullins RD, Leonetti MD, Huang B

Nanometer-accuracy distance measurements between fluorophores at the single-molecule level.

Proceedings of the National Academy of Sciences of the United States of America

Niekamp S, Sung J, Huynh W, Bhabha G, Vale RD, Stuurman N

Cellular aspect ratio and cell division mechanics underlie the patterning of cell progeny in diverse mammalian epithelia.

eLife

McKinley KL, Stuurman N, Royer LA, Schartner C, Castillo-Azofeifa D, Delling M, Klein OD, Vale RD

Visualizing Calcium Flux in Freely Moving Nematode Embryos.

Biophysical journal

Ardiel EL, Kumar A, Marbach J, Christensen R, Gupta R, Duncan W, Daniels JS, Stuurman N, Colón-Ramos D, Shroff H

Micro-Magellan: open-source, sample-adaptive, acquisition software for optical microscopy.

Nature methods

Pinkard H, Stuurman N, Corbin K, Vale R, Krummel MF

Impact of New Camera Technologies on Discoveries in Cell Biology.

The Biological bulletin

Stuurman N, Vale RD

Quantitative evaluation of software packages for single-molecule localization microscopy.

Nature methods

Sage D, Kirshner H, Pengo T, Stuurman N, Min J, Manley S, Unser M

High-resolution imaging of cardiomyocyte behavior reveals two distinct steps in ventricular trabeculation.

Development (Cambridge, England)

Staudt DW, Liu J, Thorn KS, Stuurman N, Liebling M, Stainier DY

Advanced methods of microscope control using μManager software.

Journal of biological methods

Edelstein AD, Tsuchida MA, Amodaj N, Pinkard H, Vale RD, Stuurman N

Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells.

Proceedings of the National Academy of Sciences of the United States of America

Moutinho-Pereira S, Stuurman N, Afonso O, Hornsveld M, Aguiar P, Goshima G, Vale RD, Maiato H

Chromophore-assisted laser inactivation in neural development.

Neuroscience bulletin

Li W, Stuurman N, Ou G

Biological imaging software tools.

Nature methods

Eliceiri KW, Berthold MR, Goldberg IG, Ibáñez L, Manjunath BS, Martone ME, Murphy RF, Peng H, Plant AL, Roysam B, Stuurman N, Stuurmann N, Swedlow JR, Tomancak P, Carpenter AE

Software tools, data structures, and interfaces for microscope imaging.

Cold Spring Harbor protocols

Stuurman N, Swedlow JR

Computer control of microscopes using µManager.

Current protocols in molecular biology

Edelstein A, Amodaj N, Hoover K, Vale R, Stuurman N

Polarized myosin produces unequal-size daughters during asymmetric cell division.

Science (New York, N.Y.)

Ou G, Stuurman N, D'Ambrosio M, Vale RD

Determining single-molecule intensity as a function of power density.

Cold Spring Harbor protocols

Reck-Peterson SL, Derr ND, Stuurman N

Imaging single molecules using total internal reflection fluorescence microscopy (TIRFM).

Cold Spring Harbor protocols

Reck-Peterson SL, Derr ND, Stuurman N

Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.

The Journal of cell biology

Goshima G, Mayer M, Zhang N, Stuurman N, Vale RD

Functional genomic screen reveals genes involved in lipid-droplet formation and utilization.

Nature

Guo Y, Walther TC, Rao M, Stuurman N, Goshima G, Terayama K, Wong JS, Vale RD, Walter P, Farese RV

High throughput microscopy: from raw images to discoveries.

Journal of cell science

Wollman R, Stuurman N

Genes required for mitotic spindle assembly in Drosophila S2 cells.

Science (New York, N.Y.)

Goshima G, Wollman R, Goodwin SS, Zhang N, Scholey JM, Vale RD, Stuurman N

Single-molecule observations of neck linker conformational changes in the kinesin motor protein.

Nature structural & molecular biology

Tomishige M, Stuurman N, Vale RD

Length control of the metaphase spindle.

Current biology : CB

Goshima G, Wollman R, Stuurman N, Scholey JM, Vale RD

Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen.

Proceedings of the National Academy of Sciences of the United States of America

Cheng LW, Viala JP, Stuurman N, Wiedemann U, Vale RD, Portnoy DA

Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis.

Proceedings of the National Academy of Sciences of the United States of America

Dean SO, Rogers SL, Stuurman N, Vale RD, Spudich JA

Molecular requirements for actin-based lamella formation in Drosophila S2 cells.

The Journal of cell biology

Rogers SL, Wiedemann U, Stuurman N, Vale RD

Mitochondrial positioning in fission yeast is driven by association with dynamic microtubules and mitotic spindle poles.

Proceedings of the National Academy of Sciences of the United States of America

Yaffe MP, Stuurman N, Vale RD

Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants.

Molecular plant-microbe interactions : MPMI

Stuurman N, Pacios Bras C, Schlaman HR, Wijfjes AH, Bloemberg G, Spaink HP

A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NoIL in Rhizobium leguminosarum.

Molecular plant-microbe interactions : MPMI

Pacios Bras C, Jordá MA, Wijfjes AH, Harteveld M, Stuurman N, Thomas-Oates JE, Spaink HP

Ectopic overexpression of Drosophila lamin C is stage-specific lethal.

Experimental cell research

Stuurman N, Delbecque JP, Callaerts P, Aebi U

Interactions between coiled-coil proteins: Drosophila lamin Dm0 binds to the bicaudal-D protein.

European journal of cell biology

Stuurman N, Häner M, Sasse B, Hübner W, Suter B, Aebi U

Assembly of Drosophila lamin Dm0 and C mutant proteins studied with the baculovirus system.

European journal of cell biology

Krohne G, Stuurman N, Kempf A

A tailless Drosophila lamin Dm0 fragment reveals lateral associations of dimers.

Journal of structural biology

Sasse B, Aebi U, Stuurman N

Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA.

Molecular and cellular biology

Goldberg M, Lu H, Stuurman N, Ashery-Padan R, Weiss AM, Yu J, Bhattacharyya D, Fisher PA, Gruenbaum Y, Wolfner MF

In vivo association of lamins with nucleic acids in Drosophila melanogaster.

Journal of cell science

Rzepecki R, Bogachev SS, Kokoza E, Stuurman N, Fisher PA

Nuclear lamins: their structure, assembly, and interactions.

Journal of structural biology

Stuurman N, Heins S, Aebi U

In vitro assembly of Drosophila lamin Dm0--lamin polymerization properties are conserved.

European journal of biochemistry

Sasse B, Lustig A, Aebi U, Stuurman N

Assembly of A- and B-type lamins studied in vivo with the baculovirus system.

Journal of cell science

Klapper M, Exner K, Kempf A, Gehrig C, Stuurman N, Fisher PA, Krohne G

DNA from Drosophila melanogaster beta-heterochromatin binds specifically to nuclear lamins in vitro and the nuclear envelope in situ.

Gene

Baricheva EA, Berrios M, Bogachev SS, Borisevich IV, Lapik ER, Sharakhov IV, Stuurman N, Fisher PA

Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.

Journal of cell science

Riemer D, Stuurman N, Berrios M, Hunter C, Fisher PA, Weber K

Disassembly of the Drosophila nuclear lamina in a homologous cell-free system.

Journal of cell science

Maus N, Stuurman N, Fisher PA

The t(15;17) translocation alters a nuclear body in a retinoic acid-reversible fashion.

The EMBO journal

Koken MH, Puvion-Dutilleul F, Guillemin MC, Viron A, Linares-Cruz G, Stuurman N, de Jong L, Szostecki C, Calvo F, Chomienne C

Intranuclear filaments containing a nuclear pore complex protein.

The Journal of cell biology

Cordes VC, Reidenbach S, Köhler A, Stuurman N, van Driel R, Franke WW

Induction of nuclear lamins A/C during in vitro-induced differentiation of F9 and P19 embryonal carcinoma cells.

Experimental cell research

Mattia E, Hoff WD, den Blaauwen J, Meijne AM, Stuurman N, van Renswoude J

Nuclear frameworks: concepts and operational definitions.

Cell biology international reports

Stuurman N, de Jong L, van Driel R

Three-dimensional immunogold labeling of nuclear matrix proteins in permeabilized cells.

Cell biology international reports

de Graaf A, Humbel BM, Stuurman N, van Bergen en Henegouwen PM, Verkleij AJ

Stabilization of the nuclear matrix by disulfide bridges: identification of matrix polypeptides that form disulfides.

Experimental cell research

Stuurman N, Floore A, Colen A, de Jong L, van Driel R

A monoclonal antibody recognizing nuclear matrix-associated nuclear bodies.

Journal of cell science

Stuurman N, de Graaf A, Floore A, Josso A, Humbel B, de Jong L, van Driel R

Principles of nuclear organization.

Cell biology international reports

de Jong L, van Driel R, Stuurman N, Meijne AM, van Renswoude J

The nuclear matrix from cells of different origin. Evidence for a common set of matrix proteins.

The Journal of biological chemistry

Stuurman N, Meijne AM, van der Pol AJ, de Jong L, van Driel R, van Renswoude J

The protein composition of the nuclear matrix of murine P19 embryonal carcinoma cells is differentiation-stage dependent.

Experimental cell research

Stuurman N, Van Driel R, De Jong L, Meijne AM, Van Renswoude J