Crest LFOV Spinning Disk/ C2 Confocal

This inverted Nikon Ti2-E microscope is equipped with a CREST X-Light V2 large field of view spinning disk confocal, a C2-DUVB laser scanning confocal and an FRAP unit with a 405 nm Laser for photoactivation and photobleaching.  The Crest X-Light V2 spinning has the capability of a 25mm field of view and disk is equipped with a Prime 95 25MM sCMOS camera that has a high sensitivity (95% QE) and a Celesta Light system with 7 laser lines (405, 446, 477, 520, 546, 638, 749nm). The C2-DUVB is a 4 channel (405, 488, 561, 640nm) laser scanner with a GaAsP  detector unit that can be used for spectral imaging. The microscope utilizes the Nikon Perfect Focus System, an automated piezo stage to enable multi-position high throughput imaging. It is also equipped with an Oko cage incubator and  5% CO2 making it ideal for both fixed and live specimen imaging.


Capabilities

  • 7  laser lines (405, 446, 477, 520, 546, 638, 749nm) for the spinning disk confocal
  • 4 Laser lines (405, 488, 561, 647) for the laser scanner confocal
  • Easily switch between spinning disk and laser scanner
  • 3D confocal Imaging
  • Gigapixel image stitching
  • Automated Imaging
  • Live cell time lapse imaging
  • Photobleaching and photo activation
  • Spectral Imaging

 


Objectives:

  • CFI Plan Apo Lambda 10x /0.45
  • CFI Plan Apo Lambda 20x/0.75
  • CFI Plan Apo Lambda 40x/0.95 Corr
  • CFI Plan Apo Lambda S 40x/1.25 Corr (Silicone)
  • CFI Plan Apo Lambda 60x/1.4

 

Equipment Details:

  • Nikon Ti2-E Microscope
  • Crest X-Light V2 L-FOV spinning disk confocal
  • Nikon C2 confocal with DUVb detector
  • MCL piezo stage
  • Opti-Microscan FRAP with 405nm laser line
  • Lumencor Sola SE U-NIR
  • Lumencor Celeste Light engine
  • Nikon 4-line LU-N4 Laser
  • Prime 95B 25mm sCMOS camera
  • Okolab enclosure and Bold Line incubator

Light Source:

  • Lumencor Sola SE U-NIR (Widfield/Epifluorescence)
  • Lumencor Celeste Light engine (Crest spinning disk confocal)
  • Nikon 4-line LU-N4 (C2 Confocal)

Excitation Lasers (Crest Spinning Disk Confocal):

  • 405 nm
  • 446 nm
  • 477 nm
  • 520 nm
  • 546 nm
  • 638 nm
  • 749 nm

Excitation Laser (C2 Confocal)

  • 405nm
  • 488nm
  • 561nm
  • 640nm

Emission Filters (Crest Spinning Disk Confocal):

  • Nikon Multiband
  • FF01-438/24
  • FF01-485/20
  • FF01-511/20
  • FF01-595/31
  • FF01-560/25
  • FF02-685/40
  • FF01-819/44

 

Emission Detector (C2 Confocal):

  • DUVb spectral detector