CSU-22 Spinning Disk Confocal


This microscope is an inverted Nikon Ti microscope equipped with a CSU-22 spinning disk confocal, EMCCD camera, and a cage incubator with CO2 and humidity control. The system is optimized for imaging live cells with high sensitivity and resolution. The Nikon Perfect Focus System keep live samples in focus over time and multiple positions, while HCA jobs can be programed for easy scanning of plates and samples.




  • 3D confocal imaging
  • 4-color fluorescence imaging
  • Live cell time lapse imaging
  • Automated multi-position imaging



  • Plan Flour 10x / 0.3 Ph1 
  • Plan Apo λ 40x/0.95
  • Plan Apo VC 60x / 1.2 WI
  • Plan Apo VC 60x/1.4 Oil
  • Plan Apo VC 100x / 1.4 Oil
  • Apo TIRF 100x/1.49 Oil

Equipment Details:

  • Nikon Ti Microscope (Inverted)
  • Yokogawa CSU-22 Spinning Disk Confocal
  • Custom laser launch (100 mW at 405, 488, 561, and 640 nm)
  • Photometrics Evolve Delta EMCCD camera
  • Sutter emission filter wheel
  • Prior motorized XY stage with Z piezo

Light Source:

  • Custom 4-line solid state laser launch

Excitation Wavelengths:

  • 405nm
  • 488nm
  • 561nm
  • 647nm

Emission Filters:

  • ET460/50m
  • ET525/50m
  • ET645/65m
  • ET700/75m
  • ET610/60m
  • zET405/488/561/635m