Nikon CSU-X1 Spinning Disk


This is an inverted Nikon Ti microscope equipped with a CSU-X1 spinning disk confocal, Photometrics Prime 95B sCMOS camera, and an OkoLab cage incubator with CO2 and humidity control. The system is optimized for imaging live cells with high sensitivity and resolution. The Nikon Perfect Focus System can be used to keep live samples in focus over time and at multiple positions.


  • 3D confocal imaging
  • 4-color fluorescence imaging
  • Live cell time lapse imaging
  • Automated multi-position imaging
  • Stimulation with 440nm or 630nm LEDs though brightfield light path



  • Plan Apo λ 10x / 0.45
  • Plan Apo VC 20x/0.75
  • Plan Apo λ 40x/0.95
  • Plan Apo λ 40x/1.3 oil
  • Plan Apo VC 100X/1.4
  • Apo TIRF 100x/1.49 Oil

Equipment Details:

  • Nikon Ti Microscope (Inverted)
  • Yokogawa CSU-X1 Spinning Disk Confocal
  • Vortran 4-line laser launch
  • Sutter Lambda XL
  • Photometrics Prime95B sCMOS camera
  • Andor DU897 EMCCD
  • Sutter Lambda 10-3 emission filter wheel
  • ASI motorized XY stage with Z piezo
  • OkoLab Cage Incubator
  • OkoLab CO2 Mixer

Light Source:

  • Vortran 4-line solid state laser launch


Excitation Wavelengths:

  • 405nm
  • 488nm
  • 561nm
  • 640nm

Emission Filters:

  • 460/50m
  • 525/50m
  • 630/75m
  • 690/50m