Nikon Widefield Epifluorescence

 

The Nikon Widefield Epifluorescence microscope is an inverted Nikon Ti microscope set up for 4-color fluorescence imaging for both fixed and live cells. The microscope is equipped with an Andor Clara interline CCD, an In Vivo Scientific cage incubator, and an Oko lab CO2 mixer.

 

Links

ILAB CALENDAR

FPBASE

 


Capabilities

  • Bright field and DIC imaging
  • 4-color fluorescence imaging
  • Live cell imaging
  • Automated imaging

 


Objectives:

  • Plan Apo 4x/0.2
  • Plan Fluor 10x/0.3
  • Plan Apo VC 0.75NA 20x
  • Plan Apo 20X ELWD

Equipment Details:

  • Nikon Ti  Inverted Microscope
  • Andor Clara Interline CCD
  • In Vivo Scientific Cage incubator
  • Oko CO2 Mixer
  • Sutter Lambda XL lamp

Light Source:

  • Sutter Lambda XL lamp

Excitation Filters

  • DAPI (340-380nm)
  • FITC (465-495nm) 
  • TRITC (528-553nm)
  • Cy5 (625-650nm)
  • mCherry LP (542-582nm)

Emission Filters:

  • DAPI (435-485nm)
  • FITC (515-555nm)
  • TRITC (590-650nm)
  • Cy5 (peak 670)
  • mCherry-LP (604-1100nm)