This is a conventional widefield epifluorescence system equipped with a 37°C incubation chamber and CO2 control for live cell imaging. It is set up for epifluorescence and brightfield with Quantitative label-free imaging with phase and polarization (QLIPP) imaging in collaboration with Shalin Mehta's Lab at the CZ-Biohub. Nikon Perfect Focus keeps samples in focus over hours or days for automated time-lapse experiments, while the automated stage allows for multi-position imaging

Capabilities

• Quantitative label-free imaging with phase and polarization (QLIPP) imaging (collaboration required)
• Brightfield, phase contrast, and DIC imaging
• 4-color fluorescence imaging
• Live cell imaging
• Automated time lapse imaging
• Automated multiwell plate imaging

Objectives:

• Plan Fluor 10x/0.3 (Ph1) 
• Plan Apo 20x/0.75 
• Plan Fluor 40x/0.75 
• Plan Fluor 40x/1.3 Oil
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Light Source:

• Lumencor Spextra X