Time Lapse


This is a conventional widefield epifluorescence system equipped with a 37°C incubation chamber and CO2 control for live cell imaging. Nikon Perfect Focus keeps samples in focus over hours or days for automated time-lapse experiments while the automated stage allows for multi-position imaging.


  • Brightfield, phase contrast, and DIC imaging
  • 4-color fluorescence imaging
  • Live cell imaging
  • Automated time lapse imaging
  • Automated multiwell plate imaging



  • Plan Fluor 10x/0.3 (Ph1) 
  • Plan Apo 20x/0.75 
  • Plan Fluor 40x/0.75 
  • Plan Fluor 40x/1.3 Oil
  • Plan Apo 60x/1.2 WI 
  • Plan Apo 100x/1.4 Oil (Ph3)

Equipment Details:

  • Nikon Ti Microscope (Inverted)
  • Hamamatsu Flash 4.0 with camera link
  • Sutter Lambda 821 LED light source
  • Sutter emission filter wheels
  • OkoLab Cage Incubator with CO2

Light Source:

  • Sutter Lambda 821 LED


Excitation Wavelengths and Filters infront of LED

  • 340nm (FF01-340/26-25-STR)
  • 380nm (FF01-389/38-25-STR)
  • 440nm (FF01-445/40-25-STR)
  • 480nm (FF01-480/40-25-STR)
  • 530nm (FF01-504/12-STR)
  • 561nm (FF01-562/40-25-STR)
  • 630nm (FF01-630/38-25-STR)

Emission Filters:

  • Fura2 emission filter
  • 440/11m Semrock Sedat Quad
  • 525/30m Semrock Sedat Quad
  • 607/36m Semrock Sedat Quad
  • 684/24m Semrock Sedat Quad
  • ET525/50m GFP/mCherry ET (89021 Em2)
  • ET632/60m GFP/mCherry ET (89021 Em1)
  • ET470/24m CFP/YFP (89002 Em2)
  • ET535/30m CFP/YFP (89002 Em1