This is a conventional widefield epifluorescence system equipped with a 37°C incubation chamber and CO2 control for live cell imaging. Nikon Perfect Focus keeps samples in focus over hours or days for automated time-lapse experiments while the automated stage allows for multi-position imaging.

Capabilities

• Brightfield, phase contrast, and DIC imaging
• 4-color fluorescence imaging
• Live cell imaging
• Automated time lapse imaging
• Automated multiwell plate imaging

Objectives:

• Plan Fluor 10x/0.3 (Ph1) 
• Plan Apo 20x/0.75 
• Plan Fluor 40x/0.75 
• Plan Fluor 40x/1.3 Oil
• Plan Apo 60x/1.2 WI 
• Plan Apo 100x/1.4 Oil (Ph3)

Light Source:

• Sutter Lambda 821 LED

Excitation Wavelengths and Filters infront of LED

• 340nm (FF01-340/26-25-STR)
• 380nm (FF01-389/38-25-STR)
• 440nm (FF01-445/40-25-STR)
• 480nm (FF01-480/40-25-STR)
• 530nm (FF01-504/12-STR)
• 561nm (FF01-562/40-25-STR)
• 630nm (FF01-630/38-25-STR)